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For experiments aimed at optimizing electroporation conditions, a cDNA encoding the enhanced green fluorescent protein (eGFP) gene and containing a T7 promoter and polyadenylation signal suitable for DC transfection was prepared using standard methods. For transfection into DCs, mRNA was dissolved in water at 1.0 g/mL. Electrotransfection parameters were optimized by monitoring transfection efficiency and DC viability as a function of electrode separation, pulse amplitude and length and amplified mRNA concentration in the medium (Figure 1)..

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